TITLE:
Molecular Characterization and mRNA Expression of Cytochrome P450 1A1 and Cytochrome P450 3A in Liver of Kafue Lechwe (Kobus leche kafuensis) as Potential Biomarkers of Pollution of the Kafue River Basin, Zambia
AUTHORS:
Ethel M’kandawire, Michelo Syakalima, Kaampwe Muzandu, Girja Pandey, Martin Simuunza, Shouta M. M. Nakayama, Yusuke K. Kawai, Yoshinori Ikenaka, Mayumi Ishizuka
KEYWORDS:
Lochnivar Game Management Area; Blue Lagoon Game Management Area; Pestcides/Herbicides; Biomonitoring; Phylogenetic Relationships; Real-Time RT-PCR
JOURNAL NAME:
Engineering,
Vol.6 No.2,
January
28,
2014
ABSTRACT: The aim of this
study was to characterize CYP1A1 and CYP3A in liver of the Kafue lechwe from
Lochnivar and Blue Lagoon GMAs and to determine their mRNA expression levels as
potential biomarkers of pollution. Increasing attention is paid to the problem of pesticide pollution by
people. Risk assessment of pesticide should not be only based on the chemical
analysis of environmental samples, since this method could not truly
distinguish whether the pesticide was harmful to environment. Thus, biological
assessment should be established.