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National Committee for Clinical Laboratory Standards, “Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically: Approved Standard,” 6th Edition, Clinical and Laboratory Standards Institute, Wayne, 2003.

has been cited by the following article:

• TITLE: Use of Essential Oils of the Genus Citrus as Biocidal Agents

  AUTHORS: Marcos S. Gomes, Maria das G. Cardoso, Maurilio J. Soares, Luís R. Batista, Samísia M. F. Machado, Milene A. Andrade, Camila M. O. de Azeredo, Juliana Maria Valério Resende, Leonardo M. A. Rodrigues

  KEYWORDS: Volatile Essential Oil; Trypanosoma cruzi; Gram-Negative Bactéria; Funghi; Antimicrobial

  JOURNAL NAME: American Journal of Plant Sciences, Vol.5 No.3, January 26, 2014

  ABSTRACT: In this study, the essential oils extracted from the peels of Citrus aurantifolia, Citrus limon and Citrus sinensis were chemically characterized and quantified. These essential oils and their standards limonene, citral and limonene + citral were evaluated (at concentrations ranging from 500 to 3.91 mL·mL-1) regarding their anti-trypanosome, antifungal and antibacterial activities. The chemical identification and quantification (by GC/FID and GC/MS) displayed the limonene as the major component of the three essential oils. It did not verified anti-trypanosome activity for all treatments. Regarding antimicrobial activity, the results were satisfactory against Gram-negative bacteria (Staphylococcus aureus, Listeria. monocytogenes and Salmonella cholerasuis) and funghi (Aspergillus niger, A. flavus and A. carbonarius), except for the bacterium Pseudomonas aeruginosa, which showed no inhibition for any treatment used.