TITLE:
The Stimulatory Effects of the Antimicrobial Agents Bavistin, Cefotaxime and Kanamycin on In Vitro Plant Regeneration of Centella asiatica (L.)—An Important Antijaundice Medicinal Plant
AUTHORS:
Chandra Sekhar Panathula, Manikyam Doraiswamy Naidu Mahadev, Challagundla Varadarajulu Naidu
KEYWORDS:
Centella asiatica (L.); Bavistin; Cefotaxime; Kanamycin; 6-Benzyl Amino Purine; In Vitro Regeneration
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.3,
January
26,
2014
ABSTRACT: Antimicrobial agents such as bavistin, cefotaxime
and kanamycin were evaluated for their effects on the rapid shoot regeneration from nodal explants of Centella asiatica (L.). Filter
sterilized bavistin (250 mg/L) was augmented alone and in combination with
cytokinins such as BAP and TDZ into the media to trace the effect on regeneration. On this basis, the potential use of bavistin (150 mg/L) along
with BAP (2.0 mg/L) was evaluated which showed the maximum shoot number (6.6)
and shoot length (4.4 cm) respectively. Cefotaxime at the concentration of 100 μM/L was found to be effective to obtain the maximum
shoot number formation (5.8) with the regeneration frequency (90%). Kanamycin
at the concentration of 80 μM/L induced
maximum shoot regeneration (5.12).
Kanamycin at 100 μM/L or at higher concentrations reduced the shoot
regeneration. The best rooting response was noticed when in vitro regenerated microshoots were transferred to the rooting medim which was
supplemented with IBA (2.0 mg/L). This combination generates
90% of regeneration frequency and maximum number of roots per shoot (14.2) and
high root length (4.2 cm). The
rooted plants were acclimatized and transferred to
field for survivalance. The addition of
antibiotics was found to be more effective and safer for using since their effects on regeneration were found to be negligible.