TITLE:
Use of Desirability Function Method in Optimization of Regeneration and Callus Induction of Alhagi camelorum
AUTHORS:
Maryam Honari, Hossein Askari, Mahmood Khosrowchahli
KEYWORDS:
Desirability Functions; Multiple Factors; Organogenesis; Alhagi; Callus Induction; Optimized Condition
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.3,
January
26,
2014
ABSTRACT: In this study, the desirability function approach
was used to simultaneously optimize multiple
factors. The variables
evaluated were combinations of growth regulators, media and organs in affecting
the organogenesis of Alhagi. In the first step of this study, various
experiments were carried out in order to optimize the callus induction and its
growth. The highest rate of callus induction was achieved from the leaf segment on MS medium supplemented with 3 mg·l-1 BAP + 6 mg·l-1 NAA.
Moreover, the highest rate of regeneration was recorded on MS medium supplemented with those 4 mg·l-1 BAP using for leaf segments. The
optimized condition of roots induction per shoot was observed in the 1/4 MS medium without
activated charcoal and supplemented with 0.25 mg·l-1 IAA. The well rooted plantlets were acclimatized
and successfully established into soil where 70% plantlets were survived. The data indicate that media, BAP, and NAA levels
may be manipulated to increase or decrease quality factors
chosen.