TITLE:
Investigation of Lipid Peroxidation Products and Antioxidant Enzyme Activities in End-Stage Renal Disease Patients
AUTHORS:
Bojana Kisic, Dijana Miric, Radojica Stolic, Aleksandra Ilic, Julijana Rasic, Mirjana Miric, Ilija Dragojevic
KEYWORDS:
Oxidative Stress; Hemodialysis; Ferritin; Antioxidant Enzymes; Lipid Peroxidation
JOURNAL NAME:
Advances in Chemical Engineering and Science,
Vol.4 No.1,
January
24,
2014
ABSTRACT: Background: Intravenous application of iron preparations which is a routine
treatment of anemia in hemodialysis patients with end-stage renal disease can
lead to iron overload in the body. Redox-active iron can catalyze the formation
of hydroxyl radicals and initiation of lipid peroxidation, increase oxidative
stress and speed up the development of complications in these patients. Objective: In this study, we determined the markers of lipid peroxidation,
protein oxidation and antioxidant enzyme activities (superoxide dismutase,
glutathione peroxidase, glutathione S-transferase) in serum of patients with
end-stage renal disease on hemodialysis, who had received repeated treatment of
iron supplementation. Patients and Methods: The study included 29 patients undergoing regular hemodialysis
treatment. These patients were divided into three groups according to the serum
ferritin levels: group I (serum ferritin between 100 and 300 mg/L); group II (serum ferritin between 301 and 600 mg/L), and group III (serum ferritin above 601 mg/L). Results: The serum of patients with the highest concentration of serum
ferritin and iron contained significantly higher level of lipid peroxidation
products, total hydroperoxides and malondialdehyde and advanced oxidation
protein products and the lowest concentration of sulfhydryl groups, reduced
glutathione and total antioxidant capacity. Conclusion: Based on the
obtained results, it can be concluded that iron supplementation in hemodialysis
patients and consequently body iron overload of exacerbated
oxidative stress have already been present in these patients.