TITLE:
Effects of laminin on hard tissue formation by bone marrow cells in vivo and in vitro
AUTHORS:
Masataka Yoshikawa, Hideyuki Kakigi, Takayoshi Yabuuchi, Hiroyuki Hayashi
KEYWORDS:
Laminin; Bone Marrow Cells; Scaffold; Hydroxyapatite; Osteogenesis
JOURNAL NAME:
Journal of Biomedical Science and Engineering,
Vol.7 No.1,
January
24,
2014
ABSTRACT: The
effect of laminin on hard tissue formation using rat bone marrow cells was assessed.
Rat bone marrow cells were obtained from femora of 6-week-old male Fischer 344
rats. In this in vivo examination,
porous cylindrical hydroxyapatite scaffolds with a hollow center were immersed
in 100 mg/ml laminin solution and air-dried. Rat bone marrow
cells in 200 ml culture medium at 1 × 106 cells/ml were
seeded in the scaffolds. The scaffolds were implanted into the dorsal
subcutis of 7-week-old male Fischer 344 rats for 6 weeks. The scaffolds were then removed and examined histologically.
For in vitro examinations, 1 × 105 rat bone marrow
cells in 2 ml culture medium were then cultured with the addition of dexamethasone
and laminin. Rat bone marrow cells were also cultured in laminin-coated culture
plates. In vitro examinations showed the effectiveness of laminin for
hard tissue formation from the results of biochemical and immunochemical
analysis. From the in vivo examination, laminin coating of the scaffolds induced hard tissue in the pores
with the cells. It is concluded that laminin is useful for bone formation, as
in an in vitro culture study using
bone marrow cells, in hydroxyapatite scaffolds in vivo.