TITLE:
Juvenile myelo-monocytic leukemia (JMML): No effect of granulocyte monocyte-colony stimulating factor (GM-CSF) on Wilms Tumor gene (WT1) by nested Polymerase Chain Reaction (nPCR) and flow cytometry
AUTHORS:
Sana Khan, Marie Olszewski, Wei Huang, Morris Kletzel
KEYWORDS:
Juvenile Myelo-Monocytic Leukemia; Wilms Tumor; Nested PCR; JMML; WT1; GM-CSF; nPCR
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.5 No.2,
January
24,
2014
ABSTRACT: This study was to determine whether GM-CSF induced WT1 gene expression and
to establish an association with markers of proliferation CD71+CD34+ using nPCR and flow cytometry respectively, in samples
obtained from 5 newly diagnosed JMML patients. Overtime (day 0 to day 14) there
was an insignificant difference in WT1 gene expression and CD71+CD34+ in JMML
samples when compared to peripheral blood of normal volunteers (n = 3). Our
study suggests that there is a correlation between WT1 gene expression and cellular
proliferation and that GMCSF in vitro does not create a significant difference in JMML samples.