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D’Arena, G., Cascavilla, N., Musto, P., et al. (1996) Flow cytometric characterization of CD34+ hematopoietic progenitor cells in mobilized peripheral blood and bone marrow of cancer patients. Haematologica, 81, 216-223.

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• TITLE: Juvenile myelo-monocytic leukemia (JMML): No effect of granulocyte monocyte-colony stimulating factor (GM-CSF) on Wilms Tumor gene (WT1) by nested Polymerase Chain Reaction (nPCR) and flow cytometry

  AUTHORS: Sana Khan, Marie Olszewski, Wei Huang, Morris Kletzel

  KEYWORDS: Juvenile Myelo-Monocytic Leukemia; Wilms Tumor; Nested PCR; JMML; WT1; GM-CSF; nPCR

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.5 No.2, January 24, 2014

  ABSTRACT: This study was to determine whether GM-CSF induced WT1 gene expression and to establish an association with markers of proliferation CD71+CD34+ using nPCR and flow cytometry respectively, in samples obtained from 5 newly diagnosed JMML patients. Overtime (day 0 to day 14) there was an insignificant difference in WT1 gene expression and CD71+CD34+ in JMML samples when compared to peripheral blood of normal volunteers (n = 3). Our study suggests that there is a correlation between WT1 gene expression and cellular proliferation and that GMCSF in vitro does not create a significant difference in JMML samples.