TITLE:
Vineyards genetic monitoring and Vernaccia di San Gimignano wine molecular fingerprinting
AUTHORS:
Monica Scali, Paolucci Elisa, Bigliazzi Jacopo, Cresti Mauro, Rita Vignani
KEYWORDS:
Vitis vinifera L.; SSRs Genotyping; Vernaccia di San Gimignano; Wine DNA; Molecular Traceability
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.5 No.2,
January
24,
2014
ABSTRACT: The definition of the genetic
profile of Vernaccia di San Gimignano (VSG) in the areas of production is an
essential step for both the implementation of a plan of analytical traceability
and the evaluation of the biological future potential of the same grape variety
in relation to any environmental change. The genetic variability of the VSG was
monitored by use of SSRs genotyping of a representative portion of individuals
belonging to both the productive vineyards and the germplasm collections that
represent the “mother plants” reservoir for future vineyards. 74% of the
individuals have been shown to be identical to the grapevine genotype reported
in databases as VSG truetype. In order to determine the wine varietal
composition by DNA analysis, four wine types commercialized as VSG were
DNA-tested at 14 loci SSRs. The molecular data obtained demonstrate the presence
as prevalent component of the VSG in the four wine types. All the wines
revealed the presence of minor varieties, whose presence/absence was
estimated by extrapolating the allele configuration that best matched to a
standard genotype. Molecular data allow us to exclude the presence of three
aromatic white grapevines that are not allowed by the actual production rules
(Disciplinare di Produzione).