TITLE:
Purine nucleotides and their metabolites in patients with type 1 and 2 diabetes mellitus
AUTHORS:
Wioleta Dudzinska
KEYWORDS:
Purine Nucleotides; Nucleosides; Purine Bases; Diabetes
JOURNAL NAME:
Journal of Biomedical Science and Engineering,
Vol.7 No.1,
January
24,
2014
ABSTRACT: We measured the erythrocyte
levels of principal nucleotides (ATP, ADP, AMP, GTP, GDP, GMP, IMP),
nucleosides (Ado, Guo, Ino) and Hyp with HPLC. Purine concentrations were
determined in the erythrocytes of 36 type 1 and 40 type 2 diabetic patients.
The increased dephosphorylation of adenine and guanine nucleotides, indicated
by increased Ado, Ino, Guo and Hyp concentrations as the products of purine
nucleotide degradation, suggests serious energy metabolism disruptions in
diabetes. An increase in AMP, GMP, IMP concentrations, as well as a decrease in
AEC and GEC values, points to significant alterations in erythrocyte purine
nucleotide concentration.