TITLE:
Influence of Additives on Enhanced in Vitro Shoot Multiplication of Stevia rebaudiana (Bert.)—An Important Anti Diabetic Medicinal Plant
AUTHORS:
Thulasi Muneppa Sridhar, Chenna Reddy Aswath
KEYWORDS:
Stevia rebaudiana; Nodal Explants; MS Medium; Growth Hormones; Additives; Shoot Proliferation
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.1,
January
22,
2014
ABSTRACT: The present study was designed to develop an
efficient protocol for micro propagation of S. rebaudiana from nodal explants and study the influence of additives on enhancement of
shoot proliferation. A two-step protocol
has been standardized in which, first step comprising growth hormones concentration is optimized and it was found that MS medium supplemented with 2.0 mg/l
BAP + 0.5 mg/l Kin + 0.1 mg/l
NAA turned out to be the best treatment for shoot induction. In the second
step, the best treatment for shoot induction was fortified with different
growth additives for further shoot proliferation. Among the different types of additives used, casein hydrolysate at 0.05% (w/v) was found to be most effective, resulted
with maximum of 15.0 shoots. 90% regeneration
frequency and shoot length of 6.0 cm were recorded per explant. Thus, the procedure described is a quick and reliable
method which could be applied for efficient large scale propagation, genetic
transformation assays and secondary metabolite production of Stevia.