TITLE:
Tetrazolium Test to Evaluate Stizolobium aterrimum Seeds Quality
AUTHORS:
Bruno B. Deminicis, Patricia Do R. Rodrigues, Bráulio P. Faria, Henrique D. Vieira, Antônio D. Pandolfi Filho, Guilherme S. Freitas
KEYWORDS:
Germination; Forage Legumes; Tetrazolium Test
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.1,
January
21,
2014
ABSTRACT: The purpose of this study was to verify the
efficiency of methods of pre-conditioning and concentrations of tetrazolium
solution in the evaluation of physiological quality of Stizolobium aterrimum seeds. Commercial
seeds have passed through the process of accelerated aging for
composing lots of 0, 24, 48, 96 and 144 hours, and were submitted to the
pre-conditioning: 1) Scarification
with sandpaper number 100 and immersion in water at room temperature for 18
hours, 2) Immersion in water at
95°C and maintained in the same water outside the heating at 25°C for 18 hours.
After preconditioning, the seed coats were removed and immersed in embryos
tetrazolium salt at 0.25%, 0.5% and 1% for 150 minutes and maintained in the
dark at 25°C for color development.
To compare the results obtained in the tetrazolium test, we performed
germination test, first counting and germination speed index. The method of scarification with sandpaper and later soaked in
water for 18 hours at 25°C, the most
efficient way in preconditioning of
seeds of Stizolobium aterrimum and concentration of 1.0%
tetrazolium solution for 150 minutes at 25°C, allows
evaluating the quality of seed lots
from this species.