TITLE:
Spectrophotometric Determination of Etravirine in Bulk and Pharmaceutical Formulations
AUTHORS:
D. Murali, S. V. Venkatrao, C. Rambabu
KEYWORDS:
Etravirine; 1, 2-Napthaquinone 4-Sulphonate; 3-Methyl-2-Benzothiazolinone Hydrazone
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.5 No.2,
January
17,
2014
ABSTRACT: Two
simple, rapid, sensitive, accurate, precise and economical Visible
Spectrophotometric methods have been developed for the determination of
Etravirine in pure and pharmaceutical formulations. These methods (A and B)
were based on nucleophilic substitution and oxidative coupling reactions of
Etravirine by 1,2-naphtha
quinone-4-sulphonate (NQS) in alkaline medium and 3-methyl-2-benzothiazolinone
hydrazone (MBTH) in acidic medium with the maximum absorbance at 414 nm and 635 nm respectively. Linearity was obtained in the
concentration range of 5 -30 μg/ml and
2 -10 μg/ml which was corroborated by the correlation
coefficient (r) values of 0.9995 and 0.9996 respectively. The methods developed
were validated with respect to linearity, accuracy (recovery), precision,
Sandell’s sensitivity, molar extinction coefficient and specificity. The
proposed methods are successfully applied for the determination of Etravirine
in bulk and pharmaceutical formulations and results were validated
statistically by recovery studies.