TITLE:
Quantitative Determination of Nitidine from Roots and Plant Tissue Culture Extracts of Toddalia asiatica (Linn.) Using HPTLC
AUTHORS:
Chinthala Praveena, Ciddi Veeresham
KEYWORDS:
Nitidine; HPTLC; Validation; Toddalia asiatica
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.5 No.2,
January
17,
2014
ABSTRACT: Toddalia asiatica Linn. is
an important medicinal plant belonging to the family Rutaceae. The plant is
well known for its antimalarial activity, which has been attributed to the
presence of benzophenanthridine alkaloid nitidine in the roots of plants. A
simple, rapid, sensitive, accurate, repeatable and robust HPTLC method has been
developed and validated for the quantitative determination of nitidine in the dried roots and plant tissue
culture extracts of T. asiatica.
Nitidine was estimated at 332 nm by densitometry using Silica gel 60 F254 as stationary phase and chloroform:methanol (7:1, v/v), and as mobile phase. Linearity was
observed in the concentration range of 25 -200 ng/spot for nitidine. The limit of detection and limit of
quantitation were found to be 0.026 and 0.086 ng/spot respectively for
nitidine. Developed method was validated according to the ICH guidelines with
respect to precision, accuracy, specificity and robustness. The technique has
been applied for the first time for the estimation of nitidine in roots and
plant tissue culture extracts of T.
asiatica. Statistical analysis data indicate the accuracy and reliability
of the method.