TITLE:
Effect of Soya Lecithin on Solubilization and Biodegradation of Pentachlorophenol by Anthracophyllum discolor
AUTHORS:
Mariela Bustamante, Olga Rubilar, María Cristina Diez
KEYWORDS:
Soya Lecithin; Solubility; Biodegradation; White-Rot Fungi; Pentachlorophenol
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.5 No.1,
January
17,
2014
ABSTRACT: The effect of
soya lecithin (SL) on solubilization and biodegradation of pentachlorophenol
(PCP) by Anthracophyllum discolor was evaluated. Besides, the PCP effect
on critical micelle concentration (CMC) of SL and the effect of PCP and SL on
the ligninolytic activity of A. discolor were evaluated. The PCP solubilization test was
performed for 100, 200 and 400 mg·L-1 with SL solution varying
concentrations of ranging from 0 to 0.9 g·L-1. The effect of PCP (5
to 20 mg·L-1) on CMC of SL by conductivity was evaluated. The effect
of SL (0 to 0.9 g·L-1) on biodegradation of PCP (21.4 mg·L-1)
in modified Kirk medium was studied. The results showed that SL addition
enhanced PCP solubility in water. The solubilities of 100, 200 and 400 mg·L-1 of PCP were about 4.2, 5.0
and 2.5 times higher than their solubility in pure water when the system was
added with 0.9 g·L-1 of SL. We found that the increase in PCP
concentration caused a decrease in the CMC value. In the biodegradation assay,
in liquid medium supplemented with 0.9 g·L-1 of SL, the 94.4% of PCP
was biodegraded after 26 days of incubation. In contrast, in the control assay
(without SL), the 76.5% of PCP was removed. The PCP degradation was confirmed
by the presence of its metabolites. During biodegradation assay, A. discolor mainly produced manganese peroxidase reaching a maximum of 96.8 U·g-1 (dw) when the culture
medium was added with 0.6 g·L-1 of SL. These results show that SL can be applied to increasing the bioavailability and biodegradation of PCP.