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L. M. Canfield, M. T. Clandinin, D. P. Davies, M. C. Fernandez, J. Jackson, J. Hawkes, W. J. Goldman, K. Pramuk, H. Reyes, B. Sablan, T. Sonobe and X. Bo, “Multinational Study of Major Breast Milk Carotenoids of Healthy Mothers,” European Journal of Nutrition, Vol. 42, No. 3, 2003, pp. 133-141

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• TITLE: A Method for the Measurement of Lutein in Infant Formula

  AUTHORS: Rebecca Yuhas, Megan Mccormick, Stephen Yachetti, Anita M. Burgher, Kadeline Kong, John Walsh

  KEYWORDS: Lutein, HPLC, Infant Formula, Carotenoids

  JOURNAL NAME: Food and Nutrition Sciences, Vol.2 No.2, March 31, 2011

  ABSTRACT: Lutein is an antioxidant that is deposited in the macular region of the eye and is crucial to macular function. Lutein is present in human milk and recently has been added to infant and toddler formulas. A method for the extraction and quantification of lutein from lutein-fortified infant formula and toddler milk products was developed. The lipids and carotenoids were extracted from the samples with ethanol and hexane:tetrahydrofuran. Polar xanthophylls were ex-tracted from the organic mixture with ethanol:water, leaving behind lipids and less polar carotenoids. The ethanol:water extracts were dried, reconstituted in mobile phase, and the lutein was quantified by high performance liquid chromatography with UV detection. A C30 carotenoid column and a mobile phase gradient of methyl-tert-butyl ether and methanol were used for the chromatographic separation. Validation data showed repeatability with relative stan-dard deviations