TITLE:
Polymeric Nanohydrogels of Poly(N-Isopropylacrylamide) Combined with Others Functionalized Monomers: Synthesis and Characterization
AUTHORS:
Lissette Agüero Luztonó, Yuneivy Cepero Donates, Luis Guillermo Guerrero Ramirez, Addys González Palomo, Dionisio Zaldivar Silva, Issa Katime
KEYWORDS:
N-Isopropylacrylamide; Nanohydrogels; Inverse Microemulsion Polymerization; In Vitro Biocompatibility
JOURNAL NAME:
Journal of Biomaterials and Nanobiotechnology,
Vol.5 No.1,
January
16,
2014
ABSTRACT: Nanohydrogels from inverse
microemulsion (w/o) polymerization, at 25°C, of N-isopropylacrylamide (NIPA) and functionalized
monomers are described. The
functionalized monomers were: N-(pyridine-4-ylmethyl)
acrylamide (NP4MAM) and tert-butyl 2-acrylamidoethyl
carbamate (2AAECM). The polymeric nanohydrogel obtained was characterized by
attenuated total reflectance Fourier-transformed
infrared spectroscopy (ATR-FTIR) and proton nuclear magnetic resonance spectrometry (1HNMR),
while their morphology and particle size was assessed by scanning electron
microscopy (SEM) and dynamic light scattering. Their thermal properties were
studied by Differential Scanning Calorimetry (DSC) and Thermogravimetric
Analysis (TGA). As a preliminary measure of biocompatibility, in vitro evaluations of the
nanohydrogels were carried out by cellular toxicity (colon carcinoma cells, CT-26) and hemocompatibility tests. These evaluations
showed that these nanohydrogels were not toxic in the examined concentration
range and exhibited preliminary blood compatibility; therefore they could be
used in biomedical applications.