TITLE:
The inflammatory response of the pulp after direct capping with platelet-rich plasma and enamel matrix derivative: A controlled animal study
AUTHORS:
Murat Maden, Ekim Onur Orhan, İhsan Furkan Ertuğrul, Burcu Sengüven
KEYWORDS:
Enamel Matrix Derivate; Platelet Rich Plasma; Mineral Trioxide Aggregate; Inflammatory Response; Direct Pulp Capping
JOURNAL NAME:
Open Journal of Stomatology,
Vol.4 No.1,
January
15,
2014
ABSTRACT: Aims:
To evaluate the inflammatory response of the exposed pulp of incisor teeth in
rats after direct pulp capping, using platelet rich plasma (PRP), enamel matrix
derivate (EMD), mineral trioxide aggregate (MTA) and calcium hydroxide (Ca(OH)2).
Methods: The study was conducted on 36 Wistar albino rats with a total of 144
incisor teeth. The pulps of 96 teeth of the rats were perforated and capped
with different agents. Serving as the
positive control group, the pulps of 24 teeth were only perforated and
capped without capping agents, whereas the
pulps of 24 teeth were used as the negative control group without
being perforated (without any process). The research was ended with the
extracting of the teeth on the 7th day-28th day. The teeth were taken to the
routine and histological follows; cross sections were prepared and painted
with hematoxylen & eosin. All of the sections were evaluated in terms of
inflammatory reaction by histologic analysis taken by light microscope.
Statistical analysis was used. The normal distribution of all data was tested
with the Mann Whitney U and the differences between the groups were analyze dusing
Kruskal Wallis test at 0.05 level. Results: There are no statistically
significant differences in terms of inflammation type and necrosis among the
treatment groups on 7 days’ post capping. However, improved inflammatory cell
accumulation, hyperemia and lowest necrosis were observed from the samples
treated with PRP (p
emia were higher in the 28th day (p and most
necrosis were seen in the EMD group. These new PRP materials might serve
as pulp capping biomaterials to induce initial healing response in the future.