TITLE:
Evaluation of antibiotic activity produced by Pseudomonas aeruginosa LV strain against Xanthomonas arboricola pv. pruni
AUTHORS:
Fernanda Corrêa da Silva Vasconcellos, Admilton Gonçalves de Oliveira, Lucilene Lopes-Santos, amile Priscila de Oliveira Beranger, Martha Viviana Torres Cely, Ane Stefano Simionato, Juliana Fonteque Pistori, Flavia Regina Spago, João Carlos Palazzo de Mello, Juca Abramo Barrera San Martin, Celia Guadalupe Tardeli de Jesus Andrade, Galdino Andrade
KEYWORDS:
Peach Tree; Bacterial Spot; Biological Control; Leaf Lesions; Xanthomonas arboricola Pv. pruni
JOURNAL NAME:
Agricultural Sciences,
Vol.5 No.1,
January
14,
2014
ABSTRACT: Bacterial spot caused by Xanthomonas
arboricola pv. pruni (Xap) is considered as a major problem in
peach orchards. Copper and antibiotics are used to control, and biocontrol
should be a new alternative with low environment impact. The objective was
evaluated by the antibiotic activity of the metabolite produced by Pseudomonas aeruginosa LV strain. The free cells supernatant was
fractionated with a serial organic solvent with crescent polarity and a dichloromethane phase was concentrated and lyophilised, and after was fractionated
using vacuum liquid chromatography. The antibiotic activity of the
F3 fraction containing an organometallic compound was tested on Xap in vitro and in a greenhouse
conditions. Plants were sprayed with F3 before or after Xap infection and the results showed changes in exopolysaccharides
and cell morphology. The F3 concentration of 450 μg·mL-1 was more effective. The results showed
that F3 fraction could be a new alternative to control bacterial spot.