TITLE:
A Transparent Polyimide Film as a Biological Cell Culture Sheet with Microstructures
AUTHORS:
Hirotaka Maenosono, Hirofumi Saito, Yasushiro Nishioka
KEYWORDS:
Transparent Polymide; Parylene; Polyetheretherketone; OP9 Cell; Hot Embossing
JOURNAL NAME:
Journal of Biomaterials and Nanobiotechnology,
Vol.5 No.1,
January
13,
2014
ABSTRACT: The research on stem cell cultures has attracted much
attention due to the recent development of regenerative medicine. Therefore,
higher functionalities for devices used for culturing cells are strongly
demanded. In this study, we fabricated cell culture sheets using transparent
polyimide (PI), parylene (PA), and polyetheretherketone (PEEK) to make polymer materials that had
microstructures. We then cultured stromal marrow cells (OP9) on them and
investigated the cell alignment within the microstructures. Hot embossing was
used to fabricate the microstructures with a width and depth of 5 μm on the
polymer substrates. Cultivation of the cells was confirmed on the transparent PI and PA sheets, however,
it was not observed on the PEEK sheet. Slight alignment of the cells was also
observed along with the microstructures.