TITLE:
Crocetin Prevents Amyloid β1-42-Induced Cell Death in Murine Hippocampal Cells
AUTHORS:
Yuta Yoshino, Mitsue Ishisaka, Naofumi Umigai, Masamitsu Shimazawa, Kazuhiro Tsuruma, Hideaki Hara
KEYWORDS:
Alzheimer’s Disease; Amyloid β1-42; Crocetin; HT22; Oxidative Stress
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.5 No.1,
January
13,
2014
ABSTRACT: Crocetin is an aglycon of carotenoid extracted by saffron stigmas (Crocus sativus L.) and known to have a potent anti-oxidative
effect. Amyliod β (Aβ), hallmark of Alzheimer’s disease, is
reported to have neurotoxicity partly via oxidative
stress. In this study, we investigated the effect of crocetin on hippocampal
HT22 cell death induced by Aβ1-42.
Furthermore, to clarify the mechanism underlying the protective effects of
crocetin against Aβ1-42- induced cell death, we measured reactive oxygen species (ROS) production
by CM-H2DCFDA kit assay. Crocetin at 1 -10 μM protected HT22
cells against Aβ1-42-induced
neuronal cell death and decreased ROS production increased by Aβ1-42. These results that crocetin has the potent neuroprotective effect against
Aβ1-42-induced cytotoxicity
in hippocampal cells by attenuating oxidative stress, suggest that crocetin may provide a useful therapeutic strategy against Aβ-related disorders.