TITLE:
The Application of Nutrimetabolomics to Investigating the Bioaccessibility of Nutrients in Ham Using a Batch in Vitro Digestion Model
AUTHORS:
Xiaoyan Pan, Frances Smith, Matthew T. Cliff, Francesco Capozzi, E. N. Clare Mills
KEYWORDS:
In Vitro Digestion; Ham; Nutrimetabolomics; NMR; Bioaccessibility
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.5 No.1,
January
7,
2014
ABSTRACT: Delivering high quality dietary protein at an affordable
price is a major aim of the EU-funded CHANCE project. Foods have been
formulated with this aim and as part of their nutritional assessment; the
bioaccessbility of nutrients
following simulated gastroduodenal digestion is being investigated. Nutrimetabolomics approaches can
be used to comprehensively and quantitatively analyse nutrients and
metabolites. They have been applied to monitor nutrient
release from ham, formulated in the CHANCE project, during in vitro digestion. SDS-PAGE analysis shows that constituent
ham proteins were broken down to lower molecular weight polypeptides (Mr ≤ 10 kDa) after 120 min simulated gastric digestion
which was digested further by
subsequent duodenal digestion. Digestion of
porteins resulted in the appearance of coalesced lipid droplets associated with the loss of the muscle protein matrix of the ham. Important nutrients, such
as choline, creatine, carnosine, sucrose, cholesterol, triacylglyceride and fatty acids (saturated and
unsaturated) were identified using 1H NMR. Chance ham is a good source of dietary protein and
the combined approach can provide representative data on the bioaccessibility
of all detectable nutrients contained in CHANCE ham
to human digestive system.