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Nishikawa, G., Sekine, S., Ogawa, R., Matsubara, A., Mori, T., Taniguchi, H., Kushima, R., Hiraoka, N., Tsuta, K., Tsuda, H. and Kanai, Y. (2013) Frequent GNAS mutations in low-grade appendiceal mucinous neoplasms. British Journal of Cancer 108, 951-958.
http://dx.doi.org/10.1038/bjc.2013.47

has been cited by the following article:

• TITLE: Adenocarcinomas of the gallbladder from United States patients demonstrate less frequent molecular change for several genetic markers than other intra-abdominal cancers

  AUTHORS: Peter Zauber, Stephen Marotta, Marlene Sabbath-Solitare

  KEYWORDS: Gallbladder Carcinoma; Molecular Genetic Changes; KRAS Mutation; GNAS Mutation; BRAF Mutation; Microsatellite Instability; Loss of Heterozygosity

  JOURNAL NAME: Open Journal of Gastroenterology, Vol.3 No.8, December 26, 2013

  ABSTRACT: Context: The incidence of gallbladder cancer is quite low in the US, with an estimate (2013) for new cases of less than 10,000. The rarity suggests a possible shared molecular pathology that might facilitate a greater understanding of this tumor. Objective: We wished to assess the molecular genetic profile of this tumor, particularly KRAS gene mutations, which are frequent in tumors associated with chronic inflamemation elsewhere within the abdomen. Design: We ascertained 25 cases of gallbladder adenocarcinoma from our pathology department records for 2000-2012. PCR based techniques were used to evaluate the DNA for loss of heterozygosity of the APC and DCC genes; for point mutations in the KRAS gene, codons 12 and 13; for point mutation in the BRAF gene, codon 600; for point mutation in the GNAS gene, codon 201; and for microsatellite instability. Results: Patients included 5 males and 20 females. Approximately three-quarters of cases were associated with gallstones, inflammation and dysplasia. Microsatellite instability and GNAS mutation, both present in just 4% of cases, and BRAF mutation present in no cases, do not appear to be significant parts of carcinogenesis of gallbladder carcinoma. We detected a KRAS gene mutation in only 8% of the cases. Loss of heterozygosity for the APC was detected in 16.7% of informative cases; and for the DCC gene, in 34.8% of informative cases. Conclusions: Many molecular genetic changes frequently seen with tumors arising from other intra-abdominal organs are infrequent in this tumor type. In particular, KRAS mutations were uncommon, in contra-distinction to other malignant tumors developing in the setting of chronic inflammation/infection.