TITLE:
Optimization of medium components using response surface methodology (RSM) for mycelium biomass and exopolysaccharide production by Lentinus squarrosulus
AUTHORS:
Rahayu Ahmad, Najeeb Kaid Nasser Al-Shorgani, Aidil Abdul Hamid, Wan Mohtar Wan Yusoff, Fauzi Daud
KEYWORDS:
Lentinus squarrosulus; Response Surface Methodology (RSM); Exopolysaccharide; Submerged Culture; Central Composite Design (CCD)
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.12,
December
26,
2013
ABSTRACT: The interaction between sucrose, yeast extract and initial pH was investigated to optimize critical medium components for mycelium biomass and production of exopolysaccharide (EPS) of Lentinus squarrosulus using Response Surface Methodology (RSM). A central composite design (CCD) was applied and a polynomial regression model with quadratic term was used to analyse the experimental data using analysis of variance (ANOVA). ANOVA analysis showed that the model was very significant (p Lentinus squarrosulus are as follows: sucrose concentration 114.61 g/L, yeast extract 1.62 g/L and initial pH of 5.81; sucrose concentration 115.8 g/L, yeast extract of 3.39 g/L and initial pH of 6.44 respectively.