TITLE:
Induction of Extracellular Lytic Enzymes by Fusarium solani
AUTHORS:
María de Guadalupe Moctezuma-Zárate, Juan Manuel Vargas-Morales, Juan F. Cárdenas-González, Víctor Manuel Martínez-Juárez, Ismael Acosta-Rodríguez
KEYWORDS:
Fusarium solani; Necrotrophic; Fungus; Lytic; Enzymes
JOURNAL NAME:
Advances in Microbiology,
Vol.3 No.8A,
December
26,
2013
ABSTRACT: Fusarium solani is a necrotrophic parasitic fungus that causes wilt in some plants,
causing severe economic losses in some areas of the country. The objective of
this work was to analyze the induction of extracellular lytic enzymes produced
by a strain of F. solani, isolated
from a culture of tomato, in Villa de Arista, S.L.P. México. Polygalacturonase
activity has a greater induction time at 10 days, and the xylanase has two
times higher activity at 8 and 13 days of incubation at 28?C. Also, the
xylanase activities A and B were very stable at 4?C. After 7 days of
incubation, it has an activity of 100% and 96%, respectively, while
polygalacturonase retains 61% of its initial activity. Both activities are
better induced with glutamate and urea as nitrogen sources respectively, and
both exhibit an initial pH optimum of 5.5. Finally, we didn’t find
cellulase activity in the analyzing conditions.