TITLE:
Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells
AUTHORS:
Si Nga Sou, Karen M. Polizzi, Cleo Kontoravdi
KEYWORDS:
Transient Gene Expression; Transfection Efficiency; Chinese Hamster Ovary Cells; mRNA Stability; Temperature Shift
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.12,
December
23,
2013
ABSTRACT: Three transfection reagents, Lipofectamine® 2000, TransIT-PRO® and linear 25 kDa polyethylenimine were evaluated for transient expression of enhanced green fluorescent protein in Chinese hamster ovary cells. TransIT-PRO® was found to be more efficient under the examined conditions, but comes at an increased cost compared to the widely used PEI.