TITLE:
Description of the technique of bone marrow harvesting in the coxal tuberosity for isolation and culture of mesenchymal stem cells of buffaloes (Bubalus bubalis)
AUTHORS:
Carolina Nogueira de Moraes, Amanda Jerônimo Listoni, Leandro Maia, Carla Martins de Queiroz, Flavia Caroline Destro, Eunice Oba, Fernanda da Cruz Landim-Alvarenga
KEYWORDS:
Buffaloes; MSCs; Coxal Tuberosity; Culture
JOURNAL NAME:
Stem Cell Discovery,
Vol.4 No.1,
December
20,
2013
ABSTRACT: Several studies with
mesenchymal stem cells (MSCs) have been developed in many species because of
its ability to differentiate into other mesoderm lineages, capacity of
self-regeneration, low immunogenicity, paracrine, anti-inflammatory,
immunomodulatory and antiapoptotic effects which make them a promissory source
to be used in therapeutic strategies. The aim of this study is to report the
technique of harvest of bone marrow (BM) in the coxal tuberosity (CT) of
buffaloes and its processing and
cultivation. For this, after anesthetic block from the region corresponding
to the CT, bone marrow harvesting was performed with a myelogram’s needle. The
samples collected showed plastic adherence with 96 hours and took approximately 32 days to reach 80%
confluence. And then differentiation into adipogenic and osteogenic lineages
was performed. Samples showed morphological changes during differentiation
protocol, but not all presented production of extracellular deposits of calcium
or intracellular fat droplets. The anatomical site tested showed to be
an alternative site of harvest of BM once provided with the appropriate
isolation and culture of the mononuclear fraction. Moreover, the procedure was
performed without difficulty and with great security.
Based on this, it can be concluded that CT is an excellent anatomical
site for isolation and culture of MSCs and the proposed technique is viable and
feasible to be held in buffaloes.