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Y. Yabuta, M. Tamoi, K. Yamamoto, K. Tomizawa, A. Yokota and S. Shigeoka, “Molecular Design of Photosynthesis-Elevated Chloroplasts for Mass Accumulation of a Foreign Protein,” Plant and Cell Physiology, Vol. 49, No. 3, 2008, pp. 375-385. http://dx.doi.org/10.1093/pcp/pcn014

has been cited by the following article:

• TITLE: Construction of a Shuttle Vector for Heterologous Gene Expression in Escherichia coli and Microalgae Anabaena

  AUTHORS: Donghui Song, Jing Li, Xiaoxu Hu, Bo Xi

  KEYWORDS: Anabaena sp. PCC 7120; Integrative Shuttle Vector; pAnFP; gfp Gene

  JOURNAL NAME: Engineering, Vol.5 No.10B, December 20, 2013

  ABSTRACT: Theconstruction of an integrative shuttle expression vector and potential utility was reported inEscherichiacoliandAnabaena(Nostoc) sp. strain PCC 7120. The vector comprised of the following elements: (a) an intergenic non-coding region fromAnabaenato facilitate its genomic integration (b) a strong functional PpsbAIpromoter fromAnabaenafor desired gene expression and (c) neomycin phosphotransferase gene with its own promoter for the selection of transfor-mants. The constructed vectorpAnFP was evaluated by cloning, transfer and expression of thegfpgene encoding green fluorescent protein. When theE.coliandAnabaenasp. strain PCC 7120 were transformed, intensive green fluorescence produced by the products of GFP protein was observed. This result indicated that the integrative shuttle vector pAnFP can be promisingly used in genome transformation for expression of heterologous genes inE.coliand microalgae such asAnabaenaandNostocstrains.