TITLE:
Effect of Farnesyl Caffeate-Induced Apoptosis of Lung Carcinoma Cell Line from Damage to DNA
AUTHORS:
Kyu Sik Kim, Akemi Umeyama, Toshihiro Hashimoto, Hyun-Ju Cho, Je-Jung Lee, Masao Takei
KEYWORDS:
Apoptosis; Propolis; Farnesyl Caffeate; DNA Fragmentation; Lung Cancer
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.4 No.9,
December
19,
2013
ABSTRACT: Farnesyl caffeate, synthesis of
propolis and polar bud excretion, has been reported to exhibit anti-allergic
effects in mice. However,
little is known about anti-tumor effects. In this study, we investigated the
effect of Farnesyl caffeate in cell proliferation of lung carcinoma cell line
(H157). Antiproliferative effect and apoptosis on H157 cell were evaluated
using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MTT)
and DNA fragmentation assay, respectively. Farnesyl caffeate inhibited the growth
of H157 cell in dose-dependent manner. Morphological changes of nuclei by
staining Hoechst 33258 and DNA fragmentation suggested that Farnesyl caffeate
induced death involved in a mechanism of apoptosis. Moreover, caspase-3,
caspase-7 and caspase-9 were activated by Farnesyl caffeate on H157 cell. The
protein expressions of Bax and Bcl-2 were down-regulated by Farnesyl caffeate,
resulting in cytochrome c release
from the mitochondria. Farnesyl caffeate significantly increased the expression
of p53 proteins which indicates that p53 plays a pivotal role in the initiation phase of
Farnesyl caffeate-induced HepG2 cell apoptosis. These results demonstrated
Farnesyl caffeate-induced apoptosis in human lung carcinoma cell line. More
detailed mechanism of Farnesyl caffeate-induced H157
apoptosis remains to be elucidated.