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S. Sabtcheva, T. Saga, T. Kantardjiev, M. Ivanova, Y. Ishii and M. Kaku, “Nosocomial Spread of Arma-Mediated High-Level Aminoglycoside Resistance in Enterobacteriaceae Isolates Producing CTX-M-3 Beta-Lactamase in a Cancer Hospital in Bulgaria,” Journal of Chemotherapy, Vol. 20, No. 5, 2008, pp. 593-599.

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• TITLE: Detection of 16S rRNA Methylase Genes in Gram-Negative Bacilli Isolated from Hospitals in Changchun, China

  AUTHORS: Fan Zhao, Hongyan Shi, Jinghua Li, Jiaqi Zhou, Yanbo Sun

  KEYWORDS: 16S rRNA; Methylases; Gram-Negative Bacilli

  JOURNAL NAME: Advances in Infectious Diseases, Vol.3 No.4, December 19, 2013

  ABSTRACT: Methylation of 16S rRNA is an important mechanism of aminoglycoside resistance among gram-negative pathogens. In this report, 16S rRNA methylase genes were amplified using PCR among gram-negative bacillus isolates from hospitals in the Changchun area of China and 16S rRNA methylase genotypes (armA, rmtB, rmtA, rmtC, rmtD, and npmA) were identified by direct sequencing. Fifty of the isolates (43.1%) harbored 16S rRNA methylase genes. The common 16S rRNA methylase genes were armA and rmtB (12.1% and 31.0%, respectively), whereas the rmtA, rmtC, rmtD, and npmA genes were absent from the sample. It suggests that the predominant 16S rRNA methylase genes among gramnegative bacilli in the Changchun area are armA and rmtB.