TITLE:
Detection of 16S rRNA Methylase Genes in Gram-Negative Bacilli Isolated from Hospitals in Changchun, China
AUTHORS:
Fan Zhao, Hongyan Shi, Jinghua Li, Jiaqi Zhou, Yanbo Sun
KEYWORDS:
16S rRNA; Methylases; Gram-Negative Bacilli
JOURNAL NAME:
Advances in Infectious Diseases,
Vol.3 No.4,
December
19,
2013
ABSTRACT: Methylation of 16S rRNA is an important mechanism
of aminoglycoside resistance among gram-negative pathogens. In this report, 16S rRNA methylase genes were amplified
using PCR among gram-negative bacillus isolates from hospitals in the Changchun
area of China and 16S rRNA methylase genotypes (armA, rmtB, rmtA, rmtC, rmtD, and npmA) were identified by direct sequencing.
Fifty of the isolates (43.1%) harbored 16S rRNA methylase genes. The common 16S
rRNA methylase genes were armA and rmtB (12.1% and 31.0%,
respectively), whereas the rmtA, rmtC, rmtD, and npmA
genes were absent from the sample. It suggests that the predominant 16S rRNA
methylase genes among gramnegative bacilli in the Changchun area are armA and rmtB.