TITLE:
Cloning, Characterization and Bioinformatic Analysis of the Gene Encoding the Larval Serum Protein 2 in Diapause of the Onion Maggot, Delia Antiqua
AUTHORS:
Jingjing Xu, Bin Chen, Zhengbo He, Youjin Hao
KEYWORDS:
Delia Antiqua; Diapause; Larval Serum Protein 2; Cloning; Molecular Characterization; Bioinformatic Analysis
JOURNAL NAME:
Engineering,
Vol.5 No.10B,
December
18,
2013
ABSTRACT: The full-length cDNA encoding Larval serum protein 2
(LSp-2) in the onion maggot,Delia
antiqua, was cloned and sequenced by rapid ampli?cation of cDNA ends
methods. The result showed that the cDNA was 2203 bp long and the open reading
frame (ORF) of 2106 bp encoded 701 amino acid with a calculated molecular
weight of 80.5 kDa and an isoelectric point of 5.87. The onion maggot LSp-2
shows highest homology (83%) to that ofCalliphora
vicinaat amino acid level. Its signal peptides, domains and structures were
predicted and analyzed by using bioinformatic methods. The amino acid sequence
of LSP-2 suggests that it would be a typical hexamerin.