TITLE:
Screening and Fermentation Characteristics of PHA Bacteria in Activated Sludge
AUTHORS:
Fang Yang, Rui Zhu
KEYWORDS:
PHA; Screening; Infrared Spectroscopy
JOURNAL NAME:
Engineering,
Vol.5 No.10B,
December
18,
2013
ABSTRACT: Using sludge at the bottom of Hengshui Lake as samples,
and screened out the SJ-9 strain with PHA synthesis capability
by enrichment, purification, Nile blue staining, far-infrared spectroscopy and
gas qualitative and quantitative analysis. The infrared scanning of SJ-9
bacteria product showed that this product was middle chain PHA. Optimization of
SJ-9 fermentation conditions obtained the optimal conditions with optimal
carbon source of sodium butyrate, optimal C/N of 35, optimal fermentation time
of 120 h, and optimal initial pH for culture substrate of 7.0. 10 L fermenter
amplification culture under these conditions showed consistent results
with flask culture, and the bacteria and PHA had the highest yields at 120 h,
with the maximal yields of 6.81 and 2.36 g/L, respectively.