TITLE:
A Uniaxial Loading Device for Studying Mechanoresponses of Single Plant Cell
AUTHORS:
Liqing Zhu, Xue Fu, Jie Yan, Junyu Liu
KEYWORDS:
Mechanical Loading; Plant Cell; Chamber; Feedback Control
JOURNAL NAME:
Engineering,
Vol.5 No.10B,
December
17,
2013
ABSTRACT: A system which consists of a loading chamber unit,
displacement sensor, data collector and processor, and a feedback control, was
established for applying mechanical forces to single plant cells. The method
works by compressing an agar cell-suspension block between parallel surfaces
through using a force-feedback control circuit coupled to a microchip,
delivering the pre-defined. The actual controlled stimulus is achieved whilst
measuring the force being imposed on the cell, and its deformation. TheArabidopsisprotoplasts were utilized to
test the system. It provides an experimental approach to investigate the
mechanoresponses of plant cellsin vitroconditions.