TITLE:
Isolation and Characterization of an L-Amino Acid Oxidase-Producing Marine Bacterium
AUTHORS:
Zhiliang Yu, Hua Qiao, Juanping Qiu, Peiya Xu, Peng Li
KEYWORDS:
LAAO; Pseudoalteromonas; Physiological Property; Biochemical Property; Identification
JOURNAL NAME:
Engineering,
Vol.5 No.10B,
December
17,
2013
ABSTRACT: One marine bacterial strain, R3, has been newly isolated
from the intertidal zone of Dinghai sea area. Measurements of a-keto acids and
H2O2existing in fermentation supernatant were carried
out to show that R3 can produce L-amino acid oxidase (LAAO) with a broad
substrate specificity. Physiological and biochemical analysis showed that it
can grow great at the conditions with sodium chloride concentration of 1.5%-3%,
temperature of 15?C -35?C and pH of 6-7. In
addition, molecular identification of 16S rDNA was performed to show that R3
was proximal toPseudoalteromonasspp. with the highest identity of 98.5% toPseudoalteromonasrubra. Therefore, it was designated
asPseudoalteromonassp. R3. Further
studies are required to arrive at a better understanding of this LAAO and
secure an application.