TITLE:
Genetic structure and diversity of Ukrainian red clover cultivars revealed by microsatellite markers
AUTHORS:
Yulia N. Dugar, Vitalii N. Popov
KEYWORDS:
Genetic Structure; Heterozygosity; Microsatellites; Polymorphism; Trifolium pratense L.
JOURNAL NAME:
Open Journal of Genetics,
Vol.3 No.4,
December
12,
2013
ABSTRACT: Polymorphism of microsatellite loci of Ukrainian red
clover cultivars has been studied. 87 microsatellite alleles, which occurred in
different combinations, were identified. The number of alleles ranged from 7 to
10. Microsatellite allele distribution showed that 15 alleles were common for
all the red clover cultivars (17.2%). The red clover cultivars were represented
by homozygous and heterozygous genotypes. The observed and expected heterozygosity
ranged from 0.067 to 0.269 and from 0.225 to 0.807, respectively. An analysis
of molecular variance revealed that the largest proportion of variation (68.5%)
resided at the intrapopulation level. Differentiation of the Ukrainian
cultivars was moderately expressed (FST = 0.07).