TITLE:
Molecular basis of the anti-inflammatory potential of a diarylheptanoid in murine macrophage RAW 264.7 cells
AUTHORS:
Bharathi Raja Rajaganapathy, Karthikeyan Thirugnanam, Muthusamy Velusamy Shanmuganathan, Anand Singaravelu, Lakshmi Baddireddi Subadhra
KEYWORDS:
Diarylheptanoid; NF-кB; Lipopolysaccharide; TNF-α; COX-2; iNOS; Cytokines
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.3 No.6,
December
12,
2013
ABSTRACT: Natural products play a significant role in human
health in relation to the prevention and treatment of inflammatory disorders.
In this study, we examined the molecular basis of the anti-inflammatory potential
of a diarylheptonoid (DAH) isolated from Alpinia
officinarum hexane extract (AOHE) with special emphasis on their ability to
modulate the nuclear factor-κB (NF-кB) signaling involved in the inflammatory
response. Measurement of Nitrite by Griess reaction which revealed the effect
of DAH in RAW 264.7 macrophages showed an inhibition in the nitric oxide
production through the suppression of inducible nitric oxide synthase (iNOS)
gene level expression. NF-кB reporter gene assay suggests inhibition of NF-кB
transcriptional activity, thus inhibiting LPS-induced phosphorylation and
degradation of IкBα and a downregulation of NF-кB protein expression confirms
the immunomodulatory effect of DAH. Furthermore, downregulation in the gene
level expression of NF-кB signaling markers such as IL-1β, TNF-α and COX-2
suggests the anti-inflammatory potential of DAH via inhibition of NF-кB
activation.