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Jirik, F.R., Podor, T.J., Hirano, T., Kishimoto, T., Loskutoff, D.J., Carson, D.A. and Lotz, M. (1989) Bacterial lipopolysaccharide and inflammatory mediators augment IL-6 secretion by human endothelial cells. Journal of Immunology, 142, 144-147.

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• TITLE: Molecular basis of the anti-inflammatory potential of a diarylheptanoid in murine macrophage RAW 264.7 cells

  AUTHORS: Bharathi Raja Rajaganapathy, Karthikeyan Thirugnanam, Muthusamy Velusamy Shanmuganathan, Anand Singaravelu, Lakshmi Baddireddi Subadhra

  KEYWORDS: Diarylheptanoid; NF-кB; Lipopolysaccharide; TNF-α; COX-2; iNOS; Cytokines

  JOURNAL NAME: Advances in Biological Chemistry, Vol.3 No.6, December 12, 2013

  ABSTRACT: Natural products play a significant role in human health in relation to the prevention and treatment of inflammatory disorders. In this study, we examined the molecular basis of the anti-inflammatory potential of a diarylheptonoid (DAH) isolated from Alpinia officinarum hexane extract (AOHE) with special emphasis on their ability to modulate the nuclear factor-κB (NF-кB) signaling involved in the inflammatory response. Measurement of Nitrite by Griess reaction which revealed the effect of DAH in RAW 264.7 macrophages showed an inhibition in the nitric oxide production through the suppression of inducible nitric oxide synthase (iNOS) gene level expression. NF-кB reporter gene assay suggests inhibition of NF-кB transcriptional activity, thus inhibiting LPS-induced phosphorylation and degradation of IкBα and a downregulation of NF-кB protein expression confirms the immunomodulatory effect of DAH. Furthermore, downregulation in the gene level expression of NF-кB signaling markers such as IL-1β, TNF-α and COX-2 suggests the anti-inflammatory potential of DAH via inhibition of NF-кB activation.