TITLE:
The comparison of the efficacy of swine FMD vaccine emulsified with oil adjuvant of ISA 201 VG or ISA 206 VG
AUTHORS:
Dong Li, Chunxue Zhou, Daliang She, Pinghua Li, Pu Sun, Xingwen Bai, Yingli Chen, Baoxia Xie, Zaixin Liu
KEYWORDS:
Swine FMD Vaccine; Adjuvant; ISA 201; ISA 206; Immune Efficacy
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.1 No.3,
December
12,
2013
ABSTRACT: The Seppic Company
developed a new adjuvant Montanide ISA 201
VG, the upgraded version of Montanide ISA 206 VG, which keep the advantage and
added some chemical components on the basis of ISA 206 to improve the cellular
responses. The aim of the study is to compare the efficacy of swine FMD
(foot-and-mouth) vaccine emulsified with oil adjuvant of ISA 201 or ISA 206
respectively. The pigs were vaccinated with FMD vaccine emulsified with
inactive FMD type O antigen and adjuvant ISA 201 or ISA 206 respectively,
according to 2.0 ml (1/1 dose), 0.67 ml (1/3
dose), 0.22 ml (1/9 dose) to calculate their PD50. The
sera were collected from the vaccination of the day 0, 3, 7, 14, 21, 28 and the
ELISA FMD type O antibody were detected. Furthermore, the PD50 were calculated
after the pigs were challenged with virulent
FMDV type O on 28 days post vaccination. The ELISA antibody titers of
201vaccine were significantly
higher than that of 206 (except the third time). The fifty percent of
protection dose (PD50) of 201 vaccine (PD50 = 15.59) was higher than that of 206 vaccine
(PD50 = 10.05). The above data showed that the
efficacy of the FMD vaccine emulsified with ISA 201 was better than which with
ISA 206.