TITLE:
PGJIFs, new mitochondrial PGJ2 target factors, regulate cell proliferation
AUTHORS:
Makoto Umeda, Tatsuya Uebi, Naoya Maekawa, Hiroshi Handa, Takeshi Imai
KEYWORDS:
Prostaglandin J2; Nanobeads; Mitochondria
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.1 No.3,
December
12,
2013
ABSTRACT: Our previous study
showed that prostaglandin J2 (PGJ2) interacting factor (PGJIF) was
purified and identified with magnetic nanobeads. Farther analysis of PGJ2
function shows that PGJ2 inhibits cell proliferation and rhodamine 123 incorporation. Using PGJ2- immobilized
nanobeads, two target
proteins were also purified and identified as PGJIF1 and PGJIF2. PGJIF1 genetic
analysis showed that PGJIF1 regulates
cell proliferation as well as rhodamine 123 incorporation
in mitochondria, indicating that PGJIF1 is one of the PGJ2 target proteins. The
other target protein, PGJIF2, changes its intracellular localization in
PGJ2-dependent manner. Using nanobeads technology,
two PGJ2 target factors were purified and identified.