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Fujiwara, N., Masui, A. and Imanaka, T. (1993) Purification and properties of highly thermostable alkaline protease from an alkalophilic and thermophilic Bacillus sp. Journal of Biotechnology, 30, 245-256. http://dx.doi.org/10.1016/0168-1656(93)90117-6

has been cited by the following article:

• TITLE: Optimized production and properties of thermostable alkaline protease from Bacillus subtilis SHS-04 grown on groundnut (Arachis hypogaea) meal

  AUTHORS: Folasade M. Olajuyigbe

  KEYWORDS: Alkaline Protease; Bacillus subtilis SHS-04; Groundnut (Arachis hypogaea) Meal; Low-Cost Substrate; Thermostable

  JOURNAL NAME: Advances in Enzyme Research, Vol.1 No.4, December 10, 2013

  ABSTRACT: Production of alkaline protease from Bacillus subtilis SHS-04 was investigated under different fermentation conditions involving low-cost substrates with the aim of optimizing yield of enzyme. Maximum enzyme production (1616.21 U/mL) was achieved using groundnut meal (0.75%) as nitrogen source and 0.5% glucose as carbon source at 48 h cultivation period, pH 9, 45 ° C and 200 rpm. The yield was 348% increase over comparable control samples. The alkaline protease had optimum temperature of 60 ° C and remarkably exhibited 80% relative activity at 70 ° C. It was highly thermostable showing 98.7% residual activity at 60 ° C after 60 minutes of incubation at pH 9.0 and was stable in the presence of organic solvents studied. These properties indicate the viability of the protease for biotechnological and industrial applications. The optimized yield of enzyme achieved in this study establishes groundnut meal as potential low-cost substrate for alkaline protease production by B. subtilis SHS-04.