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Sukumaran, R.K., Surender, V.J., Sindhu, R., Binod, P., Janu, K.U., Sajna, K.V., Rajasree, K.P. and Pandey, A. (2010) Lignocellu-losic ethanol in India: Prospects, challenges and feedstock availability. Bioresource Technology, 101, 4826-4833. http://dx.doi.org/10.1016/j.biortech.2009.11.049

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• TITLE: Isolation, purification and characterization of carboxymethyl cellulase (CMCase) from endophytic Fusarium oxysporum producing podophyllotoxin

  AUTHORS: Refaz Ahmad Dar, Iram Saba, Mohd Shahnawaz, Manisha Kashinath Sangale, Avinash Bapurao Ade, Shabir Ahmad Rather, Parvaiz Hassan Qazi

  KEYWORDS: Endophyte; Fusarium oxysporum; Juniperus recurva; Podophyllotoxin; CMCase

  JOURNAL NAME: Advances in Enzyme Research, Vol.1 No.4, December 10, 2013

  ABSTRACT: Endophytic fungus Fusarium oxysporum is a rich source of cellulases. In the present study, the highest activity was reported at 28 ° C, pH 5.6 with 2% Carboxymethyl cellulose (CMC) as carbon source. CMC was purified using Sephadex G and DEAE cellulose chromatography to 15.9 folds and the molecular weight was determined to be 84 kDa by SDS-PAGE analysis and was subsequently characterized. The purified enzyme was stable over the pH range from 4.0 to 8.0 and at temperatures below 50 ° C. The enzyme was highly active on CMC and reduced or no activity on Avicel, cellobiose and it was suggested to be CMCase/endoglucanase. The activity of endoglucanase was enhanced in the presence of MgCl2, CoCl2, FeCl3, CaCl2, FeCl2 and intensive to HgCl2. The purified enzyme showed its optimum activity at pH 5.0 - 6.0 and was quite stable at 50 ° C for 30 min and retained 45% of original activity.