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S. Matsuyama, M. Iwadate, M. Kondo, M. Saitoh, A. Hanyu, K. Shimizu, H. Aburatani, H. K. Mishima, T. Imamura, K. Miyazono and K. Miyazawa, “SB-431542 and Gleevec Inhibit Transforming Growth Factor-Beta-Induced Proliferation of Human Osteosarcoma Cells,” Cancer Research, Vol. 63, No. 22, 2003, pp. 7791-7798.

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• TITLE: The Knee Joint Tissues Differ Significantly in TGFβ1 Expression and Its Sensitivity

  AUTHORS: Sadanand Fulzele, Monte Hunter, Rajnikumar Sangani, Norman Chutkan, Carlos Isales, Mark W. Hamrick

  KEYWORDS: Meniscus; Outer Meniscus; Inner Meniscus; TGFβ1; Articular Cartilage

  JOURNAL NAME: CellBio, Vol.2 No.4, December 4, 2013

  ABSTRACT: The knee joint is the largest and most complex joint in the human body. In this study, we investigated TGFβ1 expression in the outer meniscus, inner meniscus and articular cartilage of rabbit and human knee tissue (outer and inner menisci) in order to determine the potential role of this factor in normal meniscal function. We also examined the potential of TGF-β1 stimulation to promote tissue regeneration in the two different regions of rabbit knee meniscus tissue. Immunohistochemical investigations of TGF-β1 were performed on rabbit and human knee tissue. The rabbit outer, inner and articular cartilage cells were culture and stimulated with TGF-β1 followed by cell proliferation assay and extracellular matrix analysis. Regulatory studies were performed using TGF-β1 inhibitors SB-431542 and PD98059. Gene expression was analyzed by quantitative polymerase chain reaction. We found marked regional variation in the expression of TGF-β1 in rabbit and human knee. TGF-β1 expressions are relatively greater in the outer meniscus than inner meniscus. Furthermore, we found that exogenous TGF-β1 stimulation increased cell proliferation and aggrecan synthesis more so in the outer than in the inner meniscus. Articular cartilage tissue shows moderate levels of cell proliferation and ECM synthesis when compared with outer and inner meniscus. These findings suggest that growth factors used to enhance the repair and regeneration of meniscal tissue should be tailored to enhance region-specific variation in cell proliferation and extracellular matrix synthesis.