TITLE:
Immunohistochemical Analysis of the Acid Secretion Potency in Gastric Parietal Cells
AUTHORS:
Rie Irie-Maezono, Shinichiro Tsuyama
KEYWORDS:
Gastric Parietal Cells; Secretory Potency; Phosphoryl Ezrin; Histochemical Morphometry
JOURNAL NAME:
CellBio,
Vol.2 No.4,
December
4,
2013
ABSTRACT: Gastric parietal cells are important in acid secretion,
but it is unclear which cells throughout the gastric gland have the highest
secretion potency. Here, we used immunohistochemical methods with anti-H+,
K+-ATPase, phosphoryl ezrin and CD44 antibodies to study the
distribution of gastric acid secretion activity. Stomach tissues from freely
fed and starved rats were cryofixed for light microscopy or fixed by
high-pressure freezing for electron microscopy. Parietal cells from freely fed
animals corresponded to the active secretion phase and to the inactive resting
phase from starved rats. Anti-H+, K+-ATPase and
anti-phosphoryl ezrin labeling were observed on the membrane of the intracellular
canaliculi and the tubulovesicle from freely fed rats, while cells from starved
animals showed weak labeling with anti-phosphoryl ezrin antibody staining. Morphometrical
analysis at the electron microscopic level was performed on active and inactive
acid secretory phases between the upper and base regions of the gland. H+,
K+-ATPase and CD44 were distributed on both sites of the
microvillous and tubulovesicle membrane in the same cells, but phosphoryl ezrin
localized predominantly on the microvillous membrane in active cells of the glandular
neck and upper base. Therefore, the highest secreting potency appeared to be in
cells of the glandular neck and upper base.