TITLE:
Aptamers—A Promising Approach for Sensing of Biothreats Using Different Bioinformatics Tools
AUTHORS:
Anamika Sharma, Rakesh Kumar Sharma
KEYWORDS:
Biosensors; Aptamers; SELEX; Insilco Designing
JOURNAL NAME:
Soft Nanoscience Letters,
Vol.3 No.4A,
December
4,
2013
ABSTRACT: Intentional release of pathogens or biotoxin against humans, plants, or animals is an impending threat all over the world. Continuous monitoring of environment is required for their detection. These signals can help to distinguish whether the bioattack has occurred or not. Biosensors utilise biological response including different biochemical reactions, antigen antibody reactions, electrochemical reactions, aptameric reactions etc. The currently available biosensors have a limit of detection, specificity and less linearity which affect their sensitivity. Aptamers are single stranded oligonucleotides binding species which are capable of tightly binding to their distinguishing targets. They are evolved from random oligonucleotides pools by using different strategies. These are capable of conscientiously distinguishing their target ligands. They have high sensitivity and a wide range of detection limit. The versatility of nucleic acid based methods allowed for the design of specific aptamer sequences, typically on the order of 10 to 30 base pairs in length, identifying the different biothreat agents in the environment. By using different bioinformatics tools we can design RNA aptamers for toxins of lectin family.