TITLE:
Development of New Strategy for Non-Antibiotic Therapy: Dromedary Camel Lactoferrin Has a Potent Antimicrobial and Immunomodulator Effects
AUTHORS:
Alaa B. Ismael, Salama M. Abd El Hafez, Manal B. Mahmoud, Abdel-Kader A. Elaraby, Hany M. Hassan
KEYWORDS:
Dromedary Camel Lactoferrin; Isolation; Antimicrobial and Immunomodulator Effects
JOURNAL NAME:
Advances in Infectious Diseases,
Vol.3 No.4,
December
2,
2013
ABSTRACT: The human and bovine lactoferrin have
been studied extensively, but very few reports have been published concerning
camel lactoferrin (cLf). The present study aimed to isolate cLf and evaluate its efficiency
including antimicrobial activity and immunomodulator effects. cLf isolation was
attempted from camel milk whey using a cation exchange chromatography by
SP-Sepharose. The
antimicrobial activity of the isolated cLf was investigated against Staphylococcus
aureus (S. aureus), Streptococcus agalactiae (S.
agalactiae), Escherichia coli (E. coli) and Pseudomonas
aeruginosa (P. aerogenosa) strains. The immune effect of cLf was
studied by lymphocyte transformation test. It was found that cLf was separated
around molecular weight of 80 kDa and showed significant inhibitory effect
against E. coli followed by P. aeruginosa, S. agalactiae and S. aureus. cLf increased lymphocyte transformations mean values in a
dose dependant manner. The highest transformations mean value was determined at
50 μg/mL. In
conclusion, these results suggest that cLf is a potent natural antimicrobial
and novel immunomodulator agent.