TITLE:
Expression of Nucleoprotein Gene of CTN Strain Rabies Virus from China in E. coli with Antigenicity
AUTHORS:
Wangbin Cao, Ying He, Qiumei Shi, Cairan Yang, Yanying Zhang
KEYWORDS:
Rabies Virus; Nucleoprotein; CTN Strain; Antigencity; Expression
JOURNAL NAME:
Open Journal of Veterinary Medicine,
Vol.3 No.7,
November
29,
2013
ABSTRACT: The nucleoprotein (NP) gene of
rabies CTN strain isolated from China was recombined into pMal-c2x. The
antigenicity of the recombined MBP-NP fusion proteins was examined by western
blotting and by enzyme linked immunosorbent assay (ELISA). The results
demonstrated that the recombined protein possesses predominant antigenicity.