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Ko, C., Dixit, V., Shaw, W., and Gitnick, G., (1995) In vitro slow release profile of endothelial cell growth factor immobi-lized within calcium alginate microbeads, Artif. Cells Blood Substit Immobil. Biotechnol., 23, 143– 151.

has been cited by the following article:

• TITLE: A new size and shape controlling method for producing calcium alginate beads with immobilized proteins

  AUTHORS: Yan Zhou, Shin’ichiro Kajiyama, Hiroshi Masuhara, Yoichiro Hosokawa, Takahiro Kaji, Kiichi Fukui

  KEYWORDS: Calcium Alginate Beads; Size Controllable Production Method; Protein Immobilized Beads; Femtosecond Laser; Laser Manipulation

  JOURNAL NAME: Journal of Biomedical Science and Engineering, Vol.2 No.5, September 17, 2009

  ABSTRACT: A method for producing size- and shape-con-trolled calcium alginate beads with immobilized proteins was developed. Unlike previous cal-cium alginate bead production methods, pro-tein-immobilized alginate beads with uniform shape and sizes less then 20 micrometers in diameter could successfully be produced by using sonic vibration. BSA and FITC-conjugated anti-BSA antibodies were used to confirm pro-tein immobilization in the alginate beads. Pro-tein diffusion from the beads could be reduced to less than 10% by cross-linking the proteins to the alginate with 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide (EDC) and N-hydroxysul-fosuccinimide (NHSS). The calcium alginate beads could also be arranged freely on a slide glass by using a femtosecond laser.