TITLE:
Dominance of Enterobacteria among Histamine-Producing Bacteria Isolated from Indian Mackerel
AUTHORS:
Meena Tembhurne, Anita Ghag, Hirekudel Sanathkumar, Binaya Bhusan Nayak
KEYWORDS:
Histamine; Scombroid Fish; Enterobacteria; 16SrDNA
JOURNAL NAME:
Advances in Microbiology,
Vol.3 No.7,
November
27,
2013
ABSTRACT: Histamine fish poisoning (HFP) is a major illness occurring
throughout the world due to the consumption of quality of deteriorated
fish containing pre-formed histamine from bacterial activities. In the study
reported here, the histamine-producing bacteria were
isolated from the muscle, gills and the gut of 19 samples of Indian mackerel (Rastrelliger kanagurta) from Mumbai,
India. The isolates from modified Niven’s medium (MNM) were confirmed for their
ability to produce histamine by using 4 different pH-indicator media,
followed by HPLC analyses. Out of 202 isolates, 63 isolates produced
considerable amounts of histamine on at least 3 out of 4 media used in this
study. The histamine formers were identified by biochemical tests followed by
sequencing of their 16SrDNA gene,
which showed that 89% of the isolates belonged to 13 different genera of the
family Enterobacteriaceae. The non-enterobacterial histamine-producing bacteria
belonged to the genera Staphylococcus, Alkaligenes, Shewanella and Psychrobacter.