TITLE:
Certification and uncertainty evaluation of flavonoids certified reference materials
AUTHORS:
Can Quan, Huanfang Yao, Caixia Hou
KEYWORDS:
Flavonoids; Certified Reference Material (CRM); Quantitative Nuclear Magnetic Resonance (QNMR); Mass balance approach; Uncertainty
JOURNAL NAME:
Agricultural Sciences,
Vol.4 No.9B,
November
27,
2013
ABSTRACT: Flavonoids are the
most widely used in the field of the food, medicine, and cosmetic due to their
pharmacological functions and biological activities, such as anti-tumor,
anti-inflammatory and antioxidation. This work described the assignment of
purity values to 3 flavonoids certified reference materials (CRMs) including
baicalein, 5,7-dihydoxy flavones and
naringenine whichwere developed in this study according to the ISO Guides 34
and 35. The qualitative analysis was performed
by liquid chromatography-mass spectrometry (LC-MS) and infrared
spectroscopy (IR). The CRMs’ purity values were assigned based on the weighted
average of quantitative nuclear magnetic resonance method and mass balance
approach with high resolution liquid chromatography-VWD. All the three CRMs
with following value mass fractions: baicalein at a certified purity P ± U (k = 2) of 98.8% ± 0.8%; 5,7-dihydoxy
flavones of 99.1% ± 0.7% and naringenine of 99.5% ± 1.0% respectively. The
homogeneity of the CRMs was determined by an in-house validated liquid
chromatographic method. Potential degradation during storage was also
investigated and a shelf-life based on this value was established.